{"id":414,"date":"2022-04-13T16:16:48","date_gmt":"2022-04-13T16:16:48","guid":{"rendered":"http:\/\/physics.uri.edu\/biophysics\/?page_id=414"},"modified":"2022-04-20T20:29:45","modified_gmt":"2022-04-20T20:29:45","slug":"phlic-technology","status":"publish","type":"page","link":"https:\/\/physics.uri.edu\/biophysics\/phlic-technology\/","title":{"rendered":"pHLIC Technology"},"content":{"rendered":"<section class=\"cl-wrapper cl-menu-wrapper\"><div id=\"\" class=\"cl-menu  \" data-name=\"research\" data-show-title=\"0\"><ul id=\"menu-research\" class=\"cl-menu-list cl-menu-list-no-js\"><li id=\"menu-item-719\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-719\"><a href=\"https:\/\/physics.uri.edu\/biophysics\/molecular-mechanism-of-membrane-associated-folding-and-unfolding\/\">Membrane Folding<\/a><\/li>\n<li id=\"menu-item-717\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-717\"><a href=\"https:\/\/physics.uri.edu\/biophysics\/phlip-technology\/\">pHLIP Technology<\/a><\/li>\n<li id=\"menu-item-718\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-718\"><a href=\"https:\/\/physics.uri.edu\/biophysics\/phlic-technology\/\">pHLIC Technology<\/a><\/li>\n<li id=\"menu-item-720\" class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-720\"><a href=\"https:\/\/physics.uri.edu\/biophysics\/protein-fluorescence\/\">Protein Fluorescence<\/a><\/li>\n<\/ul><\/div><\/section>\n\n\n\n<h1 class=\"wp-block-heading\">pHLIC Technology<\/h1>\n\n\n\n<p class=\"has-normal-font-size\"><strong>pHLIC technology<\/strong> constitutes family of pH-Low Insertion Cycles (cyclic peptides), a novel class of pH-sensitive delivery agents, which selectively target and intracellularly deliver diagnostic and therapeutic molecules to inflamed tissues including neuroinflammation. pHLICs are 8-12 aa cyclic peptides with protonatable Glu residues and Trp residues at different side of a cycle. At the normal pH, Glu residues are negatively-charged (red circles) and Trp residues (green circles) interact with headgroups of bilayer. The concentration ratio of pHLICs on inner and outer leaflets at pH<sub>surf<\/sub> = 7.4 and pH<sub>in<\/sub> = 7.2 (normal cells) is about 0.6. A drop of a pH leads to the protonation of Glu residues (blue circles), which enhances peptides hydrophobicity and induces partition into the bilayer, The concentration ratio of pHLICs on inner and outer leaflets at pH<sub>surf<\/sub> = 6.0 and pH<sub>in<\/sub> = 7.2 (metabolically active cells) is about 11 for the selected cyclic peptide.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignleft size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/physics.uri.edu\/biophysics\/wp-content\/uploads\/sites\/10\/2022\/04\/Figure-8-1024x689.jpg\" alt=\"\" class=\"wp-image-476\" width=\"600\" height=\"420\"\/><figcaption>Image is from Weerakkody et al., <em>Sci Rep.<\/em> 2016<\/figcaption><\/figure><\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>pHLIC Technology pHLIC technology constitutes family of pH-Low Insertion Cycles (cyclic peptides), a novel class of pH-sensitive delivery agents, which selectively target and intracellularly deliver diagnostic and therapeutic molecules to inflamed tissues including neuroinflammation. pHLICs are 8-12 aa cyclic peptides with protonatable Glu residues and Trp residues at different side of a cycle. At the [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-414","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/physics.uri.edu\/biophysics\/wp-json\/wp\/v2\/pages\/414","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/physics.uri.edu\/biophysics\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/physics.uri.edu\/biophysics\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/physics.uri.edu\/biophysics\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/physics.uri.edu\/biophysics\/wp-json\/wp\/v2\/comments?post=414"}],"version-history":[{"count":12,"href":"https:\/\/physics.uri.edu\/biophysics\/wp-json\/wp\/v2\/pages\/414\/revisions"}],"predecessor-version":[{"id":737,"href":"https:\/\/physics.uri.edu\/biophysics\/wp-json\/wp\/v2\/pages\/414\/revisions\/737"}],"wp:attachment":[{"href":"https:\/\/physics.uri.edu\/biophysics\/wp-json\/wp\/v2\/media?parent=414"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}